KPNA2 ELISA Kit

Produktnummer ABIN5656172

Produktdetails KPNA2 ELISA Kit

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125.00 pg/mL - 8000 pg/mL

Untere Nachweisgrenze: 125.00 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Karyopherin Alpha 2 (KPNa2). No significant cross-reactivity or interference between Karyopherin Alpha 2 (KPNa2) and analogues was observed.

Sensitivität: 53 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Alpha 2 (KPNa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Karyopherin Alpha 2 (KPNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Alpha 2 (KPNa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Alpha 2 (KPNa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Karyopherin Alpha 2 (KPNa2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Karyopherin Alpha 2 (KPNa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu KPNA2

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Andere Bezeichnung: Karyopherin Alpha 2 (KPNA2 Produkte)

Synonyme: IPOA1 ELISA Kit, QIP2 ELISA Kit, RCH1 ELISA Kit, SRP1alpha ELISA Kit, ima2 ELISA Kit, importin ELISA Kit, 2410044B12Rik ELISA Kit, PTAC58 ELISA Kit, Rch1 ELISA Kit, ipoa1 ELISA Kit, kpna2 ELISA Kit, qip2 ELISA Kit, rch1 ELISA Kit, srp1alpha ELISA Kit, hm:zeh0389 ELISA Kit, hm:zeh0389r ELISA Kit, zgc:113902 ELISA Kit, zgc:86945 ELISA Kit, karyopherin subunit alpha 2 ELISA Kit, importin subunit alpha-2 ELISA Kit, Importin subunit alpha-2 ELISA Kit, karyopherin (importin) alpha 2 ELISA Kit, karyopherin alpha-2 subunit like L homeolog ELISA Kit, karyopherin alpha 2 (RAG cohort 1, importin alpha 1) ELISA Kit, KPNA2 ELISA Kit, EDI_246290 ELISA Kit, EDI_335040 ELISA Kit, ima2 ELISA Kit, Kpna2 ELISA Kit, kpna2.L ELISA Kit, kpna2 ELISA Kit

Hintergrund:

Gene Name: Karyopherin Alpha 2

Gene Aliases: IPOA1, QIP2, RCH1, SRP1alpha, RAG Cohort 1, Importin Alpha 1, RAG cohort protein 1, SRP1-alpha

Gen-ID: 3838

UniProt: P52292

Pathways: M Phase, Protein targeting to Nucleus