HGF CLIA Kit
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- Target Alle HGF CLIA Kits anzeigen
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
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Reaktivität
- Maus
- Nachweismethode
- Chemiluminescent
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 15.62 pg/mL - 1000 pg/mL
- Untere Nachweisgrenze
- 15.62 pg/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF). No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.
- Sensitivität
- 0.63 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 - 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor (HGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor (HGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Growth Factor (HGF) level in the sample or standard.,
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle HGF CLIA Kits anzeigen
- HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
- Andere Bezeichnung
- Hepatocyte Growth Factor (HGF Produkte)
- Synonyme
- DFNB39 CLIA Kit, F-TCF CLIA Kit, HGFB CLIA Kit, HPTA CLIA Kit, SF CLIA Kit, HGF CLIA Kit, C230052L06Rik CLIA Kit, HGF/SF CLIA Kit, NK1 CLIA Kit, NK2 CLIA Kit, SF/HGF CLIA Kit, hepatocyte growth factor CLIA Kit, HGF CLIA Kit, Hgf CLIA Kit
- Hintergrund
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Gene Name: Hepatocyte Growth Factor
Gene Aliases: HGF, F-TCF, HGFB, HPTA, SF, Scatter Factor, Hepapoietin A, Fibroblast-Derived Tumor Cytotoxic Factor, Lung Fibroblast-Derived Mitogen
- Gen-ID
- 15234
- UniProt
- Q08048
- Pathways
- RTK Signalweg, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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