HSPG ELISA Kit

Produktnummer ABIN5655042

Produktdetails HSPG ELISA Kit

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Heparan Sulfate Proteoglycan (HSPG). No significant cross-reactivity or interference between Heparan Sulfate Proteoglycan (HSPG) and analogues was observed.

Sensitivität: 5.9 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate Proteoglycan (HSPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate Proteoglycan (HSPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate Proteoglycan (HSPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate Proteoglycan (HSPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparan Sulfate Proteoglycan (HSPG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparan Sulfate Proteoglycan (HSPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu HSPG

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Andere Bezeichnung: Heparan Sulfate Proteoglycan (HSPG Produkte)

Synonyme: CDW44 ELISA Kit, CSPG8 ELISA Kit, ECMR-III ELISA Kit, HCELL ELISA Kit, HUTCH-I ELISA Kit, IN ELISA Kit, LHR ELISA Kit, MC56 ELISA Kit, MDU2 ELISA Kit, MDU3 ELISA Kit, MIC4 ELISA Kit, Pgp1 ELISA Kit, AU023126 ELISA Kit, AW121933 ELISA Kit, AW146109 ELISA Kit, HERMES ELISA Kit, Ly-24 ELISA Kit, Pgp-1 ELISA Kit, CD44A ELISA Kit, METAA ELISA Kit, RHAMM ELISA Kit, CD44 molecule (Indian blood group) ELISA Kit, glypican 3 ELISA Kit, CD44 antigen ELISA Kit, CD44 ELISA Kit, GPC3 ELISA Kit, Cd44 ELISA Kit

Hintergrund:

Gene Name: Heparan Sulfate Proteoglycan

Gene Aliases: SDC2, HSPG1, SYND2, Fibroglycan, Syndecan 2, Syndecan Proteoglycan 2, Heparan Sulfate Proteoglycan 1,Cell Surface-Associated, Heparan Sulphate Proteoglycan

Gen-ID: 6383

UniProt: P34741