Gelsolin CLIA Kit

Produktnummer ABIN5654541

Produktdetails Gelsolin CLIA Kit

Target: Gelsolin (GSN)

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.91 ng/mL - 1000 ng/mL

Untere Nachweisgrenze: 3.91 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Gelsolin (GS). No significant cross-reactivity or interference between Gelsolin (GS) and analogues was observed.

Sensitivität: 0.61 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Gelsolin (GS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Gelsolin (GS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Gelsolin (GS) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gelsolin (GS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gelsolin (GS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Gelsolin (GSN)

Andere Bezeichnung: Gelsolin (GSN Produkte)

Synonyme: ADF CLIA Kit, AGEL CLIA Kit, CG1106 CLIA Kit, DGS CLIA Kit, Dmel\\CG1106 CLIA Kit, gel CLIA Kit, scin CLIA Kit, cb107 CLIA Kit, gsn CLIA Kit, sb:cb107 CLIA Kit, u-gelsolin CLIA Kit, wu:fi16f06 CLIA Kit, gelsolin CLIA Kit, Gelsolin CLIA Kit, gelsolin S homeolog CLIA Kit, gelsolin a CLIA Kit, GSN CLIA Kit, Gel CLIA Kit, Gsn CLIA Kit, gsn.S CLIA Kit, gsn CLIA Kit, gsna CLIA Kit

Hintergrund:

Gene Name: Gelsolin

Gene Aliases: GSN, AGEL, ADF, Brevin, Gelsolin Amyloidosis,Finnish Type, Actin-depolymerizing factor

Gen-ID: 2934

UniProt: P06396

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, Autophagie