GNA11 ELISA Kit

Produktnummer ABIN5654451

Produktdetails GNA11 ELISA Kit

Target: GNA11 (Guanine Nucleotide Binding Protein (G Protein), alpha 11 (Gq Class) (GNA11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of G Protein Alpha 11 (GNa11). No significant cross-reactivity or interference between G Protein Alpha 11 (GNa11) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Alpha 11 (GNa11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Alpha 11 (GNa11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Alpha 11 (GNa11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Alpha 11 (GNa11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GNA11

Target: GNA11 (Guanine Nucleotide Binding Protein (G Protein), alpha 11 (Gq Class) (GNA11))

Andere Bezeichnung: G Protein Alpha 11 (GNA11 Produkte)

Synonyme: Dsk7 ELISA Kit, E430025L19 ELISA Kit, GNA-11 ELISA Kit, HHC2 ELISA Kit, HYPOC2 ELISA Kit, G-alpha-11 ELISA Kit, gna11 ELISA Kit, si:dkey-42p8.2 ELISA Kit, wu:fb12d06 ELISA Kit, zgc:101761 ELISA Kit, guanine nucleotide binding protein, alpha 11 ELISA Kit, G protein subunit alpha 11 ELISA Kit, guanine nucleotide binding protein (G protein), alpha 11 (Gq class) ELISA Kit, guanine nucleotide regulatory protein G11 alpha subunit ELISA Kit, guanine nucleotide binding protein (G protein), alpha 11a (Gq class) ELISA Kit, guanine nucleotide binding protein (G protein), alpha 11 (Gq class) S homeolog ELISA Kit, guanine nucleotide binding protein (G protein), alpha 11b (Gq class) ELISA Kit, Gna11 ELISA Kit, GNA11 ELISA Kit, gna11 ELISA Kit, gna11a ELISA Kit, gna11.S ELISA Kit, gna11b ELISA Kit

Hintergrund:

Gene Name: G Protein Alpha 11

Gene Aliases: GA11, Guanine Nucleotide-Binding Protein Subunit Alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha

Gen-ID: 2767

UniProt: P29992

Pathways: G-protein mediated Events