FNDC5 ELISA Kit

Produktnummer ABIN5654327

Produktdetails FNDC5 ELISA Kit

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Fibronectin Type III Domain Containing Protein 5 (FNDC5). No significant cross-reactivity or interference between Fibronectin Type III Domain Containing Protein 5 (FNDC5) and analogues was observed.

Sensitivität: 6.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibronectin Type III Domain Containing Protein 5 (FNDC5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibronectin Type III Domain Containing Protein 5 (FNDC5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibronectin Type III Domain Containing Protein 5 (FNDC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibronectin Type III Domain Containing Protein 5 (FNDC5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibronectin Type III Domain Containing Protein 5 (FNDC5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FNDC5

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Andere Bezeichnung: Fibronectin Type III Domain Containing Protein 5 (FNDC5 Produkte)

Synonyme: FRCP2 ELISA Kit, irisin ELISA Kit, 1500001L03Rik ELISA Kit, AI836596 ELISA Kit, C87088 ELISA Kit, PeP ELISA Kit, Pxp ELISA Kit, fndc5 ELISA Kit, si:dkey-264p5.1 ELISA Kit, zgc:158684 ELISA Kit, fibronectin type III domain containing 5 ELISA Kit, fibronectin type III domain containing 5b ELISA Kit, FNDC5 ELISA Kit, Fndc5 ELISA Kit, fndc5b ELISA Kit

Hintergrund:

Gene Name: Fibronectin Type III Domain Containing Protein 5

Gene Aliases: FRCP2, Irisin, Fibronectin type III repeat-containing protein 2

Pathways: Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation