SLC40A1 ELISA Kit

Produktnummer ABIN5654129

Produktdetails SLC40A1 ELISA Kit

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ferroportin (FPN). No significant cross-reactivity or interference between Ferroportin (FPN) and analogues was observed.

Sensitivität: 0.131 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferroportin (FPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferroportin (FPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferroportin (FPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferroportin (FPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferroportin (FPN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferroportin (FPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SLC40A1

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Andere Bezeichnung: Ferroportin (SLC40A1 Produkte)

Synonyme: FPN1 ELISA Kit, HFE4 ELISA Kit, IREG1 ELISA Kit, MST079 ELISA Kit, MTP1 ELISA Kit, SLC11A3 ELISA Kit, slc39a1 ELISA Kit, weh ELISA Kit, slc40a1 ELISA Kit, MGC82700 ELISA Kit, SLC40A1 ELISA Kit, Fpn1 ELISA Kit, Slc11a3 ELISA Kit, Slc39a1 ELISA Kit, Dusg ELISA Kit, MTP ELISA Kit, Ol5 ELISA Kit, Pcm ELISA Kit, ARABIDOPSIS THALIANA IRON-REGULATED PROTEIN 1 ELISA Kit, ATIREG1 ELISA Kit, FERROPORTIN 1 ELISA Kit, T19C21.5 ELISA Kit, T19C21_5 ELISA Kit, iron regulated 1 ELISA Kit, ARABIDOPSIS IRON-REGULATED TRANSPORTER 1 ELISA Kit, ATIRT1 ELISA Kit, T16H5.50 ELISA Kit, T16H5_50 ELISA Kit, iron-regulated transporter 1 ELISA Kit, solute carrier family 40 member 1 ELISA Kit, solute carrier family 40 (iron-regulated transporter), member 1 ELISA Kit, solute carrier family 40 (iron-regulated transporter), member 1 S homeolog ELISA Kit, iron regulated 1 ELISA Kit, iron-regulated transporter 1 ELISA Kit, SLC40A1 ELISA Kit, slc40a1 ELISA Kit, slc40a1.S ELISA Kit, Slc40a1 ELISA Kit, IREG1 ELISA Kit, IRT1 ELISA Kit

Hintergrund:

Gene Name: Ferroportin

Gene Aliases: SLC40A1, FPN1, HFE4, IREG1, MTP1, SLC11A3, Solute Carrier Family 40,Member 1, Iron-Regulated Transporter

Gen-ID: 30061

UniProt: Q9NP59

Pathways: Transition Metal Ion Homeostasis