SLC1A3 ELISA Kit

Produktnummer ABIN5654009

Produktdetails SLC1A3 ELISA Kit

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Excitatory Amino Acid Transporter 1 (EAAT1). No significant cross-reactivity or interference between Excitatory Amino Acid Transporter 1 (EAAT1) and analogues was observed.

Sensitivität: 0.123 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 1 (EAAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 1 (EAAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 1 (EAAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 1 (EAAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 1 (EAAT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 1 (EAAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SLC1A3

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Andere Bezeichnung: Excitatory Amino Acid Transporter 1 (SLC1A3 Produkte)

Synonyme: AF001784 ELISA Kit, CG3747 ELISA Kit, Dglt-1 ELISA Kit, Dglut-1 ELISA Kit, Dmel\\CG3747 ELISA Kit, EAAT ELISA Kit, EAAT1 ELISA Kit, dEAAT ELISA Kit, dEAAT1 ELISA Kit, dEaat1 ELISA Kit, slc1a3 ELISA Kit, wu:fb98e05 ELISA Kit, wu:fd50b05 ELISA Kit, zgc:56661 ELISA Kit, zgc:77768 ELISA Kit, SLC1A3 ELISA Kit, GLAST ELISA Kit, GluT-1 ELISA Kit, EA6 ELISA Kit, GLAST1 ELISA Kit, AI504299 ELISA Kit, B430115D02Rik ELISA Kit, Eaat1 ELISA Kit, GLAST-1 ELISA Kit, GLU-T ELISA Kit, Gmt1 ELISA Kit, MGluT1 ELISA Kit, Excitatory amino acid transporter 1 ELISA Kit, solute carrier family 1 member 3 ELISA Kit, excitatory amino acid transporter 1 ELISA Kit, solute carrier family 1 (glial high affinity glutamate transporter), member 3a ELISA Kit, solute carrier family 1 (glial high affinity glutamate transporter), member 3 ELISA Kit, Eaat1 ELISA Kit, SLC1A3 ELISA Kit, PTRG_00379 ELISA Kit, Tsp_02392 ELISA Kit, slc1a3a ELISA Kit, Slc1a3 ELISA Kit

Hintergrund:

Gene Name: Excitatory Amino Acid Transporter 1

Gene Aliases: SLC1A3, EA6, GLAST1, Sodium-dependent glutamate/aspartate transporter 1, Solute Carrier 1 Member 3,Glial High Affinity Glutamate Transporter, GLutamate Aspartate Transporter

Pathways: Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport