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ESR2 ELISA Kit

ESR2 Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5653991
  • Target Alle ESR2 ELISA Kits anzeigen
    ESR2 (Estrogen Receptor 2 (ESR2))
    Reaktivität
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.
    Sensitivität
    0.117 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Beta (ERb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Beta (ERb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ESR2 ELISA Kits anzeigen
    ESR2 (Estrogen Receptor 2 (ESR2))
    Andere Bezeichnung
    Estrogen Receptor Beta (ESR2 Produkte)
    Synonyme
    ER-BETA ELISA Kit, ESR-BETA ELISA Kit, ESRB ELISA Kit, ESTRB ELISA Kit, Erb ELISA Kit, NR3A2 ELISA Kit, ER[b]1 ELISA Kit, zfER-beta1 ELISA Kit, zfER[b]1 ELISA Kit, erb2 ELISA Kit, CERB ELISA Kit, ERB ELISA Kit, erb ELISA Kit, esrb ELISA Kit, estrb ELISA Kit, nr3a2 ELISA Kit, ERbeta ELISA Kit, er-beta ELISA Kit, esr-beta ELISA Kit, ESR2 ELISA Kit, ERBETA ELISA Kit, er ELISA Kit, ER-beta ELISA Kit, ER[b] ELISA Kit, Estrb ELISA Kit, ER[b]2 ELISA Kit, ER[b]a ELISA Kit, zfER[b]2 ELISA Kit, zfER-beta2 ELISA Kit, Erb2 ELISA Kit, esr2 ELISA Kit, erbeta ELISA Kit, Esr2 ELISA Kit, ERBeta2 ELISA Kit, estrogen receptor 2 ELISA Kit, estrogen receptor 2b ELISA Kit, estrogen receptor beta 2 ELISA Kit, estrogen receptor 2 (beta) ELISA Kit, estrogen receptor 2a ELISA Kit, estrogen receptor beta ELISA Kit, ESR2 ELISA Kit, esr2b ELISA Kit, erb2 ELISA Kit, esr2 ELISA Kit, Esr2 ELISA Kit, esr2a ELISA Kit, erbeta ELISA Kit
    Hintergrund

    Gene Name: Estrogen Receptor Beta

    Gene Aliases: ER-B, ESRB, ESTRB, NR3A2, NR3-A2, ESR2, Estrogen Receptor 2, Nuclear Receptor Subfamily 3,Group A,Member 2

    Gen-ID
    2100
    UniProt
    Q92731
    Pathways
    Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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