EPO ELISA Kit (Erythropoietin)

Details for Product EPO ELISA Kit No. ABIN5653969, Anbieter: Anmelden zum Anzeigen
Antigen
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • erythropoietin S homeolog
  • erythropoietin a
  • EPO
  • epo
  • epo.S
  • Epo
  • epoa
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
24
23
23
13
11
9
6
6
5
4
4
3
3
2
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
31.25 pg/mL - 2000 pg/mL
Untere Nachweisgrenze
31.25 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Proben Cell Lysate, Tissue Homogenate
Analytische Methode Quantitative
Nachweismethode Chemiluminescent
Spezifität This assay has high sensitivity and excellent specificity for detection of Erythropoietin (EPO). No significant cross-reactivity or interference between Erythropoietin (EPO) and analogues was observed.
Sensitivität 1.2 pg/mL
Andere Bezeichnung Erythropoietin (EPO ELISA Kit Abstract)
Hintergrund

Gene Name: Erythropoietin

Gene Aliases: EP, Epoetin, Erythropoetin, Hematopoietin, Hemopoietin

Gen-ID 2056
UniProt P01588
Pathways JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
Kommentare

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer 2 - 3 h
Plattentyp Pre-coated
Protokoll The microplate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Erythropoietin (EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Erythropoietin (EPO) level in the sample or standard.,
Testpräzision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Haltbarkeit 4-8 months
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