ELAVL1 ELISA Kit
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- Target Alle ELAVL1 ELISA Kits anzeigen
- ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of ELAV Like Protein 1 (ELAVL1). No significant cross-reactivity or interference between ELAV Like Protein 1 (ELAVL1) and analogues was observed.
- Sensitivität
- 0.117 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELAV Like Protein 1 (ELAVL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ELAV Like Protein 1 (ELAVL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELAV Like Protein 1 (ELAVL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ELAV Like Protein 1 (ELAVL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle ELAVL1 ELISA Kits anzeigen
- ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))
- Andere Bezeichnung
- ELAV Like Protein 1 (ELAVL1 Produkte)
- Synonyme
- ELAV1 ELISA Kit, HUR ELISA Kit, Hua ELISA Kit, MelG ELISA Kit, HuR ELISA Kit, 2410055N02Rik ELISA Kit, W91709 ELISA Kit, elra ELISA Kit, fa25f08 ELISA Kit, fb59a02 ELISA Kit, fc60g02 ELISA Kit, hua ELISA Kit, wu:fa25f08 ELISA Kit, wu:fb59a02 ELISA Kit, wu:fc60g02 ELISA Kit, zHuA ELISA Kit, Elavl1 ELISA Kit, Xel-1 ELISA Kit, elav1 ELISA Kit, elrA ELISA Kit, hur ELISA Kit, melg ELISA Kit, p36 ELISA Kit, ElrA ELISA Kit, elavl1 ELISA Kit, elavl1-a ELISA Kit, elavl1-b ELISA Kit, ELAV like RNA binding protein 1 ELISA Kit, ELAV-like protein 1 ELISA Kit, ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) ELISA Kit, ELAV like RNA binding protein 1a ELISA Kit, ELAV like RNA binding protein 1 L homeolog ELISA Kit, ELAV like RNA binding protein 1 S homeolog ELISA Kit, ELAVL1 ELISA Kit, Elavl1 ELISA Kit, elav1 ELISA Kit, elavl1a ELISA Kit, elavl1.L ELISA Kit, elavl1.S ELISA Kit
- Hintergrund
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Gene Name: ELAV Like Protein 1
Gene Aliases: ELAV1, HUR, Hua, MelG, Hu Antigen R, Embryonic Lethal,Abnormal Vision Like 1
- Pathways
- AMPK Signaling
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