DIO2 ELISA Kit

Produktnummer ABIN5653602

Produktdetails DIO2 ELISA Kit

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Deiodinase, Iodothyronine, Type II (DIO2). No significant cross-reactivity or interference between Deiodinase, Iodothyronine, Type II (DIO2) and analogues was observed.

Sensitivität: 0.24 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deiodinase, Iodothyronine, Type II (DIO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deiodinase, Iodothyronine, Type II (DIO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Andere Bezeichnung: Deiodinase, Iodothyronine, Type II (DIO2 Produkte)

Synonyme: 5DII ELISA Kit, D2 ELISA Kit, DIOII ELISA Kit, SelY ELISA Kit, TXDI2 ELISA Kit, AI324267 ELISA Kit, Deio2 ELISA Kit, cb684 ELISA Kit, DIO2 ELISA Kit, dio2-A ELISA Kit, 5dii ELISA Kit, sely ELISA Kit, txdi2 ELISA Kit, iodothyronine deiodinase 2 ELISA Kit, deiodinase, iodothyronine, type II ELISA Kit, deiodinase, iodothyronine, type 2 L homeolog ELISA Kit, deiodinase, iodothyronine, type 2 ELISA Kit, type II iodothyronine deiodinase ELISA Kit, DIO2 ELISA Kit, Dio2 ELISA Kit, dio2 ELISA Kit, dio2.L ELISA Kit, LOC100861220 ELISA Kit

Hintergrund:

Gene Name: Deiodinase, Iodothyronine, Type II

Gene Aliases: 5DII, D2, SelY, TXDI2, Thyroxine Deiodinase,Type II, Type-II 5'-deiodinase

Gen-ID: 65162

UniProt: P70551

Pathways: Hormone Transport, Hormone Activity