CYP27B1 ELISA Kit

Produktnummer ABIN5653502

Produktdetails CYP27B1 ELISA Kit

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 27B1 (CYP27B1). No significant cross-reactivity or interference between Cytochrome P450 27B1 (CYP27B1) and analogues was observed.

Sensitivität: 0.235 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27B1 (CYP27B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27B1 (CYP27B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27B1 (CYP27B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 27B1 (CYP27B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CYP27B1

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Andere Bezeichnung: Cytochrome P450 27B1 (CYP27B1 Produkte)

Synonyme: cp2b ELISA Kit, cyp1 ELISA Kit, cyp1alpha ELISA Kit, cyp27b ELISA Kit, p450c1 ELISA Kit, pddr ELISA Kit, vdd1 ELISA Kit, vddr ELISA Kit, vddri ELISA Kit, vdr ELISA Kit, CP2B ELISA Kit, CYP1 ELISA Kit, CYP1alpha ELISA Kit, CYP27B ELISA Kit, P450c1 ELISA Kit, PDDR ELISA Kit, VDD1 ELISA Kit, VDDR ELISA Kit, VDDRI ELISA Kit, VDR ELISA Kit, Cyp40 ELISA Kit, Cp2b ELISA Kit, Cyp1 ELISA Kit, Cyp27b ELISA Kit, Pddr ELISA Kit, Vdd1 ELISA Kit, Vddr ELISA Kit, VddrI ELISA Kit, Vdr ELISA Kit, cytochrome P450 family 27 subfamily B member 1 ELISA Kit, cytochrome P450, family 27, subfamily B, polypeptide 1 ELISA Kit, cytochrome P450, family 27, subfamily b, polypeptide 1 ELISA Kit, cyp27b1 ELISA Kit, CYP27B1 ELISA Kit, Cyp27b1 ELISA Kit

Hintergrund:

Gene Name: Cytochrome P450 27B1

Gene Aliases: VDD1, PDDR, CYP1, P450c1, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase, Cytochrome P450 Family 27 Subfamily B Polypeptide 1, Calcidiol 1-monooxygenase, VD3 1A hydroxylase

Gen-ID: 13115

UniProt: O35084

Pathways: Metabolism of Steroid Hormones and Vitamin D