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CYP26A1 ELISA Kit

CYP26A1 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5653500
  • Target Alle CYP26A1 ELISA Kits anzeigen
    CYP26A1 (Cytochrome P450, Family 26, Subfamily A, Polypeptide 1 (CYP26A1))
    Reaktivität
    • 3
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 26A1 (CYP26A1). No significant cross-reactivity or interference between Cytochrome P450 26A1 (CYP26A1) and analogues was observed.
    Sensitivität
    0.055 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 26A1 (CYP26A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 26A1 (CYP26A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 26A1 (CYP26A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 26A1 (CYP26A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 26A1 (CYP26A1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 26A1 (CYP26A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CYP26A1 ELISA Kits anzeigen
    CYP26A1 (Cytochrome P450, Family 26, Subfamily A, Polypeptide 1 (CYP26A1))
    Andere Bezeichnung
    Cytochrome P450 26A1 (CYP26A1 Produkte)
    Synonyme
    CP26 ELISA Kit, CYP26 ELISA Kit, P450RAI ELISA Kit, P450RAI1 ELISA Kit, Cyp26 ELISA Kit, P450RA ELISA Kit, RAH ELISA Kit, RACE ELISA Kit, XCYP26A1 ELISA Kit, cyp26 ELISA Kit, xCYP26 ELISA Kit, cp26 ELISA Kit, p450rai ELISA Kit, p450rai1 ELISA Kit, CYP26A ELISA Kit, cb24 ELISA Kit, id:ibd5061 ELISA Kit, wu:fb81e05 ELISA Kit, cytochrome P450 family 26 subfamily A member 1 ELISA Kit, cytochrome P450, family 26, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450 family 26 subfamily A member 1 S homeolog ELISA Kit, cytochrome P450 family 26 subfamily A member 1 L homeolog ELISA Kit, cytochrome P450, family 26, subfamily A, polypeptide 1 ELISA Kit, CYP26A1 ELISA Kit, Cyp26a1 ELISA Kit, cyp26a1.S ELISA Kit, cyp26a1.L ELISA Kit, cyp26a1 ELISA Kit
    Hintergrund

    Gene Name: Cytochrome P450 26A1

    Gene Aliases: CP26, CYP26, P450RAI, P450RAI1, hP450RAI, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome

    Gen-ID
    1592
    UniProt
    O43174
    Pathways
    Retinoic Acid Receptor Signaling Pathway, Monocarboxylic Acid Catabolic Process
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