Coronin 1a ELISA Kit
-
- Target Alle Coronin 1a (CORO1A) ELISA Kits anzeigen
- Coronin 1a (CORO1A) (Coronin, Actin Binding Protein, 1A (CORO1A))
-
Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Coronin 1A (CORO1A). No significant cross-reactivity or interference between Coronin 1A (CORO1A) and analogues was observed.
- Sensitivität
- 0.113 ng/mL
- Featured
- Zu unserem meistverkauften CORO1A ELISA Kit
- Top Product
- Discover our top product CORO1A ELISA Kit
-
-
- Kommentare
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coronin 1A (CORO1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coronin 1A (CORO1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coronin 1A (CORO1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coronin 1A (CORO1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coronin 1A (CORO1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coronin 1A (CORO1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
-
- Target Alle Coronin 1a (CORO1A) ELISA Kits anzeigen
- Coronin 1a (CORO1A) (Coronin, Actin Binding Protein, 1A (CORO1A))
- Andere Bezeichnung
- Coronin 1A (CORO1A Produkte)
- Synonyme
- CORO1A ELISA Kit, cor1a ELISA Kit, Clabp ELISA Kit, Lmb3 ELISA Kit, TACO ELISA Kit, p57 ELISA Kit, CLABP ELISA Kit, CLIPINA ELISA Kit, HCORO1 ELISA Kit, fj83g12 ELISA Kit, wu:fj83g12 ELISA Kit, zgc:63766 ELISA Kit, coronin 1A ELISA Kit, Coronin-1A ELISA Kit, coronin, actin binding protein 1A ELISA Kit, coronin, actin binding protein, 1A ELISA Kit, CORO1A ELISA Kit, cor1a ELISA Kit, Coro1a ELISA Kit, coro1a ELISA Kit
- Hintergrund
-
Gene Name: Coronin 1A
Gene Aliases: CLABP, p57, CLIPINA, HCORO1, TACO, Coronin-1, Coronin,Actin-Binding Protein,1A, Coronin-like protein A, Coronin-like protein p57, Tryptophan aspartate-containing coat protein
- Gen-ID
- 11151
- UniProt
- P31146
- Pathways
- Regulation of Actin Filament Polymerization
-