CTGF CLIA Kit

Produktnummer ABIN5653292

Produktdetails CTGF CLIA Kit

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Sensitivität: 5.23 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Connective Tissue Growth Factor (CTGF) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Andere Bezeichnung: Connective Tissue Growth Factor (CTGF Produkte)

Synonyme: Ccn2 CLIA Kit, Fisp12 CLIA Kit, Hcs24 CLIA Kit, fisp-12 CLIA Kit, CCN2 CLIA Kit, HCS24 CLIA Kit, IGFBP8 CLIA Kit, NOV2 CLIA Kit, CTGRP CLIA Kit, ctgf CLIA Kit, hm:zeh1559 CLIA Kit, si:dkey-266k12.4 CLIA Kit, wu:fl25c03 CLIA Kit, zeh1559 CLIA Kit, zgc:136644 CLIA Kit, XCTGF CLIA Kit, ccn2 CLIA Kit, nov2 CLIA Kit, hcs24 CLIA Kit, igfbp8 CLIA Kit, CTGF CLIA Kit, si:ch211-173b8.2 CLIA Kit, connective tissue growth factor CLIA Kit, connective tissue growth factor a CLIA Kit, connective tissue growth factor L homeolog CLIA Kit, connective tissue growth factor b CLIA Kit, Ctgf CLIA Kit, CTGF CLIA Kit, ctgfa CLIA Kit, ctgf.L CLIA Kit, ctgf CLIA Kit, ctgfb CLIA Kit

Hintergrund:

Gene Name: Connective Tissue Growth Factor

Gene Aliases: CCN2, HCS24, IGFBP8, NOV2, Insulin-Like Growth Factor-Binding Protein 8, Hypertrophic Chondrocyte-Specific Protein 24, CCN Family Member 2

Gen-ID: 14219

UniProt: P29268

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding