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C4B ELISA Kit

C4b Reaktivität: Maus Colorimetric Sandwich ELISA 15.62 ng/mL - 1000 ng/mL Plasma, Serum
Produktnummer ABIN5653238
  • Target Alle C4B (C4b) ELISA Kits anzeigen
    C4B (C4b) (Complement Component C4b (C4b))
    Reaktivität
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.62 ng/mL - 1000 ng/mL
    Untere Nachweisgrenze
    15.62 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Complement Component 4b (C4b). No significant cross-reactivity or interference between Complement Component 4b (C4b) and analogues was observed.
    Sensitivität
    6.7 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4b (C4b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 4b (C4b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 4b (C4b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 4b (C4b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 4b (C4b) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 4b (C4b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle C4B (C4b) ELISA Kits anzeigen
    C4B (C4b) (Complement Component C4b (C4b))
    Andere Bezeichnung
    Complement Component 4b (C4b Produkte)
    Synonyme
    C4B1 ELISA Kit, C4B12 ELISA Kit, C4B2 ELISA Kit, C4B3 ELISA Kit, C4B5 ELISA Kit, C4BD ELISA Kit, C4B_2 ELISA Kit, C4F ELISA Kit, CH ELISA Kit, CO4 ELISA Kit, CPAMD3 ELISA Kit, C4 ELISA Kit, C4A2 ELISA Kit, C4A3 ELISA Kit, C4A4 ELISA Kit, C4A6 ELISA Kit, C4AD ELISA Kit, C4S ELISA Kit, CPAMD2 ELISA Kit, RG ELISA Kit, wu:fi14a03 ELISA Kit, C4B ELISA Kit, DKFZP469I0332 ELISA Kit, Slp ELISA Kit, Ss ELISA Kit, C4-2 ELISA Kit, C4l ELISA Kit, complement C4B (Chido blood group) ELISA Kit, complement C4A (Rodgers blood group) ELISA Kit, complement component 4 ELISA Kit, complement C4-B ELISA Kit, complement component 4B (Chido blood group) ELISA Kit, complement component 4A (Rodgers blood group) ELISA Kit, C4B ELISA Kit, C4A ELISA Kit, c4 ELISA Kit, LOC462577 ELISA Kit, C4a ELISA Kit, C4b ELISA Kit
    Hintergrund

    Gene Name: Complement Component 4b

    Gene Aliases: CPAMD3, C4F, CO4, C4B1, C4B3, CH, C4(Chido Blood Group), Basic complement C4, C4a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

    UniProt
    P01029
    Pathways
    Komplementsystem
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