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CD4 ELISA Kit

CD4 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5653018
  • Target Alle CD4 ELISA Kits anzeigen
    CD4
    Reaktivität
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 4 (CD4). No significant cross-reactivity or interference between Cluster Of Differentiation 4 (CD4) and analogues was observed.
    Sensitivität
    0.063 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 4 (CD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 4 (CD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 4 (CD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 4 (CD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 4 (CD4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 4 (CD4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CD4 ELISA Kits anzeigen
    CD4
    Andere Bezeichnung
    Cluster Of Differentiation 4 (CD4 Produkte)
    Synonyme
    cd4 ELISA Kit, L3T4 ELISA Kit, Ly-4 ELISA Kit, CD4mut ELISA Kit, W3/25 ELISA Kit, p55 ELISA Kit, CD4 molecule ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit, T-cell surface glycoprotein CD4 ELISA Kit, CD4 antigen ELISA Kit, Cd4 molecule ELISA Kit, CD4 ELISA Kit, LOC100125525 ELISA Kit, cd4 ELISA Kit, Cd4 ELISA Kit
    Hintergrund

    Gene Name: Cluster Of Differentiation 4

    Gene Aliases: CD4mut, p55, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

    Gen-ID
    920
    UniProt
    P01730
    Pathways
    T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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