Choline Acetyltransferase CLIA Kit

Produktnummer ABIN5652911

Produktdetails Choline Acetyltransferase CLIA Kit

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Choline Acetyltransferase (ChAT). No significant cross-reactivity or interference between Choline Acetyltransferase (ChAT) and analogues was observed.

Sensitivität: 5.92 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Choline Acetyltransferase (ChAT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Choline Acetyltransferase (ChAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Choline Acetyltransferase (ChAT) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Choline Acetyltransferase (ChAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Choline Acetyltransferase (ChAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: Choline Acetyltransferase (CHAT Produkte)

Synonyme: CHOACTASE CLIA Kit, CMS1A CLIA Kit, CMS1A2 CLIA Kit, ChoAcTase CLIA Kit, B230380D24Rik CLIA Kit, choline O-acetyltransferase CLIA Kit, choline O-acetyltransferase a CLIA Kit, choline acetyltransferase CLIA Kit, carnitine O-acetyltransferase CLIA Kit, CHAT CLIA Kit, chata CLIA Kit, Chat CLIA Kit, PGTG_00551 CLIA Kit, LOC100533322 CLIA Kit

Hintergrund:

Gene Name: Choline Acetyltransferase

Gene Aliases: CMS1A, CMS1A2, CHOACTase, Choline acetylase

Pathways: Skeletal Muscle Fiber Development