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CDC42 ELISA Kit

CDC42 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5652760
  • Target Alle CDC42 ELISA Kits anzeigen
    CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))
    Reaktivität
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cell Division Cycle Protein 42 (CDC42). No significant cross-reactivity or interference between Cell Division Cycle Protein 42 (CDC42) and analogues was observed.
    Sensitivität
    0.064 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Division Cycle Protein 42 (CDC42). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Division Cycle Protein 42 (CDC42). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Division Cycle Protein 42 (CDC42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Division Cycle Protein 42 (CDC42) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Division Cycle Protein 42 (CDC42) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Division Cycle Protein 42 (CDC42) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CDC42 ELISA Kits anzeigen
    CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))
    Andere Bezeichnung
    Cell Division Cycle Protein 42 (CDC42 Produkte)
    Synonyme
    CDC42Hs ELISA Kit, G25K ELISA Kit, AI747189 ELISA Kit, AU018915 ELISA Kit, cdc42a ELISA Kit, wu:fb07d08 ELISA Kit, wu:fj62g06 ELISA Kit, zgc:63918 ELISA Kit, Xcdc42 ELISA Kit, cdc42hs ELISA Kit, g25k ELISA Kit, CDC42 ELISA Kit, CG12530 ELISA Kit, Cdc42Dm ELISA Kit, D-CDC42 ELISA Kit, D-Cdc42 ELISA Kit, D-cdc42 ELISA Kit, DCDC42 ELISA Kit, DCdc42 ELISA Kit, Dcdc42 ELISA Kit, Dm Cdc42 ELISA Kit, DmCDC42 ELISA Kit, Dmcdc42 ELISA Kit, Dmel\\CG12530 ELISA Kit, cdc42 ELISA Kit, cell division cycle 42 ELISA Kit, Cell division control protein 42 homolog ELISA Kit, cell division control protein 42 homolog ELISA Kit, cell division cycle 42 L homeolog ELISA Kit, CG12530 gene product from transcript CG12530-RC ELISA Kit, Rho family GTPase ELISA Kit, CDC42 ELISA Kit, Cdc42 ELISA Kit, cdc42 ELISA Kit, LOC100636735 ELISA Kit, LOC100637699 ELISA Kit, LOC100638834 ELISA Kit, cdc42.L ELISA Kit
    Hintergrund

    Gene Name: Cell Division Cycle Protein 42

    Gene Aliases: CDC42Hs, G25K, G25K GTP-binding protein

    Gen-ID
    998
    UniProt
    P60953
    Pathways
    MAPK Signalweg, Microtubule Dynamics, RTK Signalweg, WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling
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