Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Caspase 8 ELISA Kit

CASP8 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5652663
  • Target Alle Caspase 8 (CASP8) ELISA Kits anzeigen
    Caspase 8 (CASP8)
    Reaktivität
    • 8
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Caspase 8 (CASP8). No significant cross-reactivity or interference between Caspase 8 (CASP8) and analogues was observed.
    Sensitivität
    0.113 ng/mL
    Featured
    Zu unserem meistverkauften CASP8 ELISA Kit
    Top Product
    Discover our top product CASP8 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 (CASP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 (CASP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8 (CASP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 8 (CASP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Caspase 8 (CASP8) ELISA Kits anzeigen
    Caspase 8 (CASP8)
    Andere Bezeichnung
    Caspase 8 (CASP8 Produkte)
    Synonyme
    casp8-A ELISA Kit, xCaspase-8 ELISA Kit, casp8 ELISA Kit, CASP8 ELISA Kit, CASP-8 ELISA Kit, FLICE ELISA Kit, MACH ELISA Kit, Mch5 ELISA Kit, ALPS2B ELISA Kit, CAP4 ELISA Kit, Casp-8 ELISA Kit, MCH5 ELISA Kit, caspase-8 ELISA Kit, zgc:92075 ELISA Kit, caspase 8 L homeolog ELISA Kit, death related ced-3/Nedd2-like protein ELISA Kit, caspase 8 ELISA Kit, caspase-8 ELISA Kit, caspase-8-like ELISA Kit, caspase 8, apoptosis-related cysteine peptidase ELISA Kit, casp8.L ELISA Kit, LOC661095 ELISA Kit, CASP8 ELISA Kit, LOC5563818 ELISA Kit, casp8 ELISA Kit, LOC100222284 ELISA Kit, Casp8 ELISA Kit
    Hintergrund

    Gene Name: Caspase 8

    Gene Aliases: CAP4, FLICE, MACH, MCH5, Cysteinyl Aspartate Specific Proteinases 8, Apoptotic cysteine protease, FADD-homologous ICE/ced-3-like protease, MORT1-associated ced-3 homolog

    Gen-ID
    64044
    UniProt
    Q9JHX4
    Pathways
    Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
Sie sind hier:
Kundenservice