Caspase 3 ELISA Kit

Produktnummer ABIN5652652

Produktdetails Caspase 3 ELISA Kit

Target: Caspase 3 (CASP3)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3). No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.

Sensitivität: 0.244 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 3 (CASP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 3 (CASP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 3 (CASP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 3 (CASP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase 3 (CASP3 Produkte)

Synonyme: CPP32 ELISA Kit, CPP32B ELISA Kit, SCA-1 ELISA Kit, A830040C14Rik ELISA Kit, AC-3 ELISA Kit, Apopain ELISA Kit, CC3 ELISA Kit, Caspase-3 ELISA Kit, Lice ELISA Kit, Yama ELISA Kit, mldy ELISA Kit, xcpp32 ELISA Kit, casp3 ELISA Kit, zgc:100890 ELISA Kit, CASP-3 ELISA Kit, caspase-3 ELISA Kit, caspase 3 ELISA Kit, caspase 3 S homeolog ELISA Kit, caspase 3, apoptosis-related cysteine peptidase a ELISA Kit, caspase 3, apoptosis-related cysteine peptidase ELISA Kit, CASP3 ELISA Kit, Casp3 ELISA Kit, casp3.S ELISA Kit, casp3a ELISA Kit

Hintergrund:

Gene Name: Caspase 3

Gene Aliases: CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1

Gen-ID: 12367

UniProt: P70677

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation