SLC25A20 ELISA Kit

Produktnummer ABIN5652610

Produktdetails SLC25A20 ELISA Kit

Target: SLC25A20 (Solute Carrier Family 25 (Carnitine/acylcarnitine Translocase), Member 20 (SLC25A20))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Carnitine Acylcarnitine Translocase (CACT). No significant cross-reactivity or interference between Carnitine Acylcarnitine Translocase (CACT) and analogues was observed.

Sensitivität: 0.235 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Acylcarnitine Translocase (CACT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Acylcarnitine Translocase (CACT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Acylcarnitine Translocase (CACT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnitine Acylcarnitine Translocase (CACT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnitine Acylcarnitine Translocase (CACT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnitine Acylcarnitine Translocase (CACT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SLC25A20

Target: SLC25A20 (Solute Carrier Family 25 (Carnitine/acylcarnitine Translocase), Member 20 (SLC25A20))

Andere Bezeichnung: Carnitine Acylcarnitine Translocase (SLC25A20 Produkte)

Synonyme: 5848 ELISA Kit, BG:DS02740.15 ELISA Kit, CACT ELISA Kit, CG5848 ELISA Kit, Cact ELISA Kit, Dmel\\CG5848 ELISA Kit, cac ELISA Kit, dip6 ELISA Kit, fs(2)ltoRN48 ELISA Kit, n(2)k17003 ELISA Kit, cact ELISA Kit, dif-1 ELISA Kit, SLC25A20 ELISA Kit, DKFZp468F1219 ELISA Kit, zgc:77760 ELISA Kit, PRKAR2A ELISA Kit, CAC ELISA Kit, 1110007P09Rik ELISA Kit, C78826 ELISA Kit, mCAC ELISA Kit, cactus ELISA Kit, solute carrier family 25 (carnitine/acylcarnitine translocase), member 20 ELISA Kit, solute carrier family 25 member 20 ELISA Kit, solute carrier family 25 (carnitine/acylcarnitine translocase), member 20 L homeolog ELISA Kit, solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20 ELISA Kit, cact ELISA Kit, slc25a20 ELISA Kit, SLC25A20 ELISA Kit, Slc25a20 ELISA Kit, slc25a20.L ELISA Kit

Hintergrund:

Gene Name: Carnitine Acylcarnitine Translocase

Gene Aliases: SLC25A20, CAC, Solute Carrier Family 25 Member 20, Carnitine Acylcarnitine Carrier, Mitochondrial carnitine/acylcarnitine carrier protein

Gen-ID: 788

UniProt: O43772

Pathways: T-Zell Rezeptor Signalweg, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades