Calmodulin 1 ELISA Kit

Produktnummer ABIN5652505

Produktdetails Calmodulin 1 ELISA Kit

Target: Calmodulin 1 (Calm1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Calmodulin (CAM). No significant cross-reactivity or interference between Calmodulin (CAM) and analogues was observed.

Sensitivität: 0.072 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calmodulin (CAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calmodulin (CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calmodulin (CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calmodulin (CAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calmodulin (CAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calmodulin (CAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Calmodulin 1

Target: Calmodulin 1 (Calm1)

Andere Bezeichnung: Calmodulin (Calm1 Produkte)

Synonyme: AI256814 ELISA Kit, AI327027 ELISA Kit, AI461935 ELISA Kit, AL024000 ELISA Kit, CaM ELISA Kit, Calm ELISA Kit, CaMI ELISA Kit, CALML2 ELISA Kit, CAMI ELISA Kit, CPVT4 ELISA Kit, DD132 ELISA Kit, LQT14 ELISA Kit, PHKD ELISA Kit, caM ELISA Kit, calm1 ELISA Kit, calm2-a ELISA Kit, calm2-b ELISA Kit, calm2a ELISA Kit, calml2 ELISA Kit, cami ELISA Kit, dd132 ELISA Kit, 3909 ELISA Kit, CAM ELISA Kit, CG8472 ELISA Kit, Cal ELISA Kit, Cal49A ELISA Kit, CalA ELISA Kit, Cam1 ELISA Kit, D-CaM ELISA Kit, DmCaM ELISA Kit, DmCalm ELISA Kit, Dmel\CG8472 ELISA Kit, anon-EST:Posey59 ELISA Kit, cam ELISA Kit, dCaM ELISA Kit, l(2)03909 ELISA Kit, l(2)3909 ELISA Kit, lincRNA.S2634 ELISA Kit, calmodulin 1 ELISA Kit, calmodulin 2 ELISA Kit, calmodulin 1 (phosphorylase kinase, delta) L homeolog ELISA Kit, Calmodulin ELISA Kit, Calm1 ELISA Kit, Calm2 ELISA Kit, CALM1 ELISA Kit, calm1.L ELISA Kit, Cam ELISA Kit

Hintergrund:

Gene Name: Calmodulin

Gene Aliases: CALcium MODULated ProteIN

Gen-ID: 801

UniProt: P62158

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction, BCR Signaling