CACNA1S ELISA Kit

Produktnummer ABIN5652487

Produktdetails CACNA1S ELISA Kit

Target: CACNA1S (Calcium Channel, Voltage-Dependent, L Type, alpha 1S Subunit (CACNA1S))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S) and analogues was observed.

Sensitivität: 0.101 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CACNA1S

Target: CACNA1S (Calcium Channel, Voltage-Dependent, L Type, alpha 1S Subunit (CACNA1S))

Andere Bezeichnung: Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNA1S Produkte)

Synonyme: CACNA1S ELISA Kit, cav1.1 ELISA Kit, ca(v)1.1 ELISA Kit, CACNL1A3 ELISA Kit, CCHL1A3 ELISA Kit, Cav1.1 ELISA Kit, HOKPP ELISA Kit, HOKPP1 ELISA Kit, MHS5 ELISA Kit, TTPP1 ELISA Kit, hypoPP ELISA Kit, AW493108 ELISA Kit, Cchl1a3 ELISA Kit, fmd ELISA Kit, mdg ELISA Kit, sj ELISA Kit, caV1.1 ELISA Kit, calcium voltage-gated channel subunit alpha1 S ELISA Kit, calcium channel, voltage-dependent, L type, alpha 1S subunit S homeolog ELISA Kit, calcium channel, voltage-dependent, L type, alpha 1S subunit ELISA Kit, CACNA1S ELISA Kit, cacna1s.S ELISA Kit, Cacna1s ELISA Kit

Hintergrund:

Gene Name: Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit

Gene Aliases: CACNL1A3, CCHL1A3, Cav1.1, HOKPP, MHS5, hypoPP, DHPR, Dihydropyridine Receptor, Voltage-gated calcium channel subunit alpha Cav1.1

UniProt: Q02789

Pathways: Skeletal Muscle Fiber Development