CAN ELISA Kit

Produktnummer ABIN5652469

Produktdetails CAN ELISA Kit

Target: CAN (Calcineurin (CAN))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Calcineurin (CaN). No significant cross-reactivity or interference between Calcineurin (CaN) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin (CaN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcineurin (CaN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcineurin (CaN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcineurin (CaN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcineurin (CaN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcineurin (CaN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CAN

Target: CAN (Calcineurin (CAN))

Andere Bezeichnung: Calcineurin (CAN Produkte)

Synonyme: CALN ELISA Kit, CALNA ELISA Kit, CALNA1 ELISA Kit, CCN1 ELISA Kit, CNA1 ELISA Kit, PPP2B ELISA Kit, CAN A1/PP2B ELISA Kit, CG1455 ELISA Kit, Can ELISA Kit, Cana1 ELISA Kit, CnA ELISA Kit, CnnA21EF ELISA Kit, D14 ELISA Kit, DmCanA1-100B ELISA Kit, Dmel\\CG1455 ELISA Kit, PP2B ELISA Kit, PP2B 21EF ELISA Kit, PpD14 ELISA Kit, canA ELISA Kit, pMY4 ELISA Kit, 2900074D19Rik ELISA Kit, AI841391 ELISA Kit, AW413465 ELISA Kit, CN ELISA Kit, Caln ELISA Kit, Calna ELISA Kit, Calna1 ELISA Kit, protein phosphatase 3 catalytic subunit alpha ELISA Kit, Calcineurin A1 ELISA Kit, protein phosphatase 3, catalytic subunit, alpha isoform ELISA Kit, PPP3CA ELISA Kit, CanA1 ELISA Kit, Ppp3ca ELISA Kit

Hintergrund:

Gene Name: Calcineurin

Gene Aliases: PPP3CA, PPP3-CA, CCN1, CNA1, CALN, CALNA1, PP2B, PP2-B, Protein Phosphatase 3(formerly 2B),Catalytic Subunit,Alpha Isoformprotein Phosphatase 2B, Calcineurin A Alpha