BMP4 CLIA Kit

Produktnummer ABIN5652268

Produktdetails BMP4 CLIA Kit

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reaktivität: Huhn

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 4 (BMP4). No significant cross-reactivity or interference between Bone Morphogenetic Protein 4 (BMP4) and analogues was observed.

Sensitivität: 5.15 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Andere Bezeichnung: Bone Morphogenetic Protein 4 (BMP4 Produkte)

Synonyme: BMP2B CLIA Kit, BMP2B1 CLIA Kit, MCOPS6 CLIA Kit, OFC11 CLIA Kit, ZYME CLIA Kit, Bmp-4 CLIA Kit, Bmp2b CLIA Kit, Bmp2b-1 CLIA Kit, Bmp2b1 CLIA Kit, BOMPR4A CLIA Kit, bmp-4 CLIA Kit, zbmp-4 CLIA Kit, zgc:100779 CLIA Kit, BMP-4 CLIA Kit, XBMP-4 CLIA Kit, bmp2b CLIA Kit, bmp2b1 CLIA Kit, bmp4 CLIA Kit, ofc11 CLIA Kit, xbmp4 CLIA Kit, zyme CLIA Kit, BMP4 CLIA Kit, bone morphogenetic protein 4 CLIA Kit, bone morphogenetic protein 4 L homeolog CLIA Kit, bone morphogenetic protein 4 S homeolog CLIA Kit, BMP4 CLIA Kit, Bmp4 CLIA Kit, bmp4 CLIA Kit, bmp4.L CLIA Kit, bmp4.S CLIA Kit

Hintergrund:

Gene Name: Bone Morphogenetic Protein 4

Gene Aliases: BMP2B, BMP2-B, BMP2B1, ZYME, DVR4, Bone morphogenetic protein 2B

UniProt: Q90752

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development