BCL11A ELISA Kit

Produktnummer ABIN5652133

Produktdetails BCL11A ELISA Kit

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 11A (Bcl11A). No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 11A (Bcl11A) and analogues was observed.

Sensitivität: 0.109 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 11A (Bcl11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 11A (Bcl11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu BCL11A

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Andere Bezeichnung: B-Cell CLL/Lymphoma 11A (BCL11A Produkte)

Synonyme: 2810047E18Rik ELISA Kit, BCL-11A ELISA Kit, Ctip1 ELISA Kit, D930021L15Rik ELISA Kit, Evi9 ELISA Kit, Evi9a ELISA Kit, Evi9b ELISA Kit, Evi9c ELISA Kit, mKIAA1809 ELISA Kit, BCL11A-L ELISA Kit, BCL11A-S ELISA Kit, BCL11A-XL ELISA Kit, BCL11a-M ELISA Kit, CTIP1 ELISA Kit, EVI9 ELISA Kit, HBFQTL5 ELISA Kit, ZNF856 ELISA Kit, bcl11a ELISA Kit, wu:fi09f10 ELISA Kit, zgc:136898 ELISA Kit, B cell CLL/lymphoma 11A (zinc finger protein) ELISA Kit, B-cell CLL/lymphoma 11A ELISA Kit, B-cell CLL/lymphoma 11A L homeolog ELISA Kit, B-cell CLL/lymphoma 11Aa ELISA Kit, Bcl11a ELISA Kit, BCL11A ELISA Kit, bcl11a.L ELISA Kit, bcl11aa ELISA Kit

Hintergrund:

Gene Name: B-Cell CLL/Lymphoma 11A

Gene Aliases: BCL11A-L, BCL11A-S, BCL11A-XL, CTIP1, EVI9, Ecotropic Viral Integration Site 9, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 homolog, Zinc finger protein 856

Gen-ID: 53335

UniProt: Q9H165

Pathways: Regulation of Cell Size