Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

ATG7 ELISA Kit

ATG7 Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5652122
  • Target Alle ATG7 ELISA Kits anzeigen
    ATG7 (ATG7 Autophagy Related 7 (ATG7))
    Reaktivität
    • 2
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 7 (ATG7). No significant cross-reactivity or interference between Autophagy Related Protein 7 (ATG7) and analogues was observed.
    Sensitivität
    0.055 ng/mL
    Featured
    Zu unserem meistverkauften ATG7 ELISA Kit
    Top Product
    Discover our top product ATG7 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 7 (ATG7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 7 (ATG7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 7 (ATG7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 7 (ATG7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 7 (ATG7) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 7 (ATG7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ATG7 ELISA Kits anzeigen
    ATG7 (ATG7 Autophagy Related 7 (ATG7))
    Andere Bezeichnung
    Autophagy Related Protein 7 (ATG7 Produkte)
    Synonyme
    APG7-LIKE ELISA Kit, APG7L ELISA Kit, GSA7 ELISA Kit, D8 ELISA Kit, UBA1B ELISA Kit, UBE1L ELISA Kit, UBE2 ELISA Kit, Ube1l ELISA Kit, 1300004C08Rik ELISA Kit, AO090001000744 ELISA Kit, atg7 ELISA Kit, 1810013K23Rik ELISA Kit, Agp7 ELISA Kit, Apg7l ELISA Kit, Atg7l ELISA Kit, ATAPG7 ELISA Kit, ATATG7 ELISA Kit, ATG7 ELISA Kit, AUTOPHAGY 7 ELISA Kit, AUTOPHAGY-RELATED 7 ELISA Kit, K15I22.10 ELISA Kit, K15I22_10 ELISA Kit, autophagy related 7 ELISA Kit, ubiquitin like modifier activating enzyme 7 ELISA Kit, ubiquitin-like modifier activating enzyme 7 ELISA Kit, autophagy-related protein 7 ELISA Kit, Autophagy-related protein 7 ELISA Kit, Apg7p ELISA Kit, ThiF family protein ELISA Kit, ubiquitin-like conjugating enzyme Atg7 ELISA Kit, ATG7 ELISA Kit, UBA7 ELISA Kit, Uba7 ELISA Kit, ANI_1_3368024 ELISA Kit, AOR_1_1338164 ELISA Kit, PTRG_02236 ELISA Kit, PAAG_00287 ELISA Kit, PITG_12537 ELISA Kit, atg7 ELISA Kit, Atg7 ELISA Kit, APG7 ELISA Kit
    Hintergrund

    Gene Name: Autophagy Related Protein 7

    Gene Aliases: APG7L, GSA7, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Ubiquitin-activating enzyme E1-like protein

    Gen-ID
    74244
    UniProt
    Q9D906
    Pathways
    Response to Water Deprivation, Autophagie
Sie sind hier:
Kundenservice