ATP2A2 ELISA Kit

Produktnummer ABIN5652085

Produktdetails ATP2A2 ELISA Kit

Target: ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ATP2A2

Target: ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))

Andere Bezeichnung: ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2 Produkte)

Synonyme: atp2b ELISA Kit, ca-p60a ELISA Kit, dar ELISA Kit, serca2 ELISA Kit, ATP2A2 ELISA Kit, ATP2B ELISA Kit, DAR ELISA Kit, DD ELISA Kit, SERCA2 ELISA Kit, SERCA2A ELISA Kit, ATP2 ELISA Kit, Serca2 ELISA Kit, SercaII ELISA Kit, 9530097L16Rik ELISA Kit, D5Wsu150e ELISA Kit, SERCA2B ELISA Kit, mKIAA4195 ELISA Kit, atp2a2 ELISA Kit, zgc:55380 ELISA Kit, ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 S homeolog ELISA Kit, ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 ELISA Kit, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 ELISA Kit, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a ELISA Kit, atp2a1.S ELISA Kit, ATP2A2 ELISA Kit, Atp2a2 ELISA Kit, atp2a2a ELISA Kit

Hintergrund:

Gene Name: ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2

Gene Aliases: ATP2B, DAR, SERCA2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process