TAP1 ELISA Kit
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- Target Alle TAP1 ELISA Kits anzeigen
- TAP1 (Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Antigen Peptide Transporter 1 (TAP1). No significant cross-reactivity or interference between Antigen Peptide Transporter 1 (TAP1) and analogues was observed.
- Sensitivität
- 0.134 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Antigen Peptide Transporter 1 (TAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Antigen Peptide Transporter 1 (TAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Antigen Peptide Transporter 1 (TAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Antigen Peptide Transporter 1 (TAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antigen Peptide Transporter 1 (TAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antigen Peptide Transporter 1 (TAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle TAP1 ELISA Kits anzeigen
- TAP1 (Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1))
- Andere Bezeichnung
- Antigen Peptide Transporter 1 (TAP1 Produkte)
- Synonyme
- ABC17 ELISA Kit, ABCB2 ELISA Kit, APT1 ELISA Kit, D6S114E ELISA Kit, PSF-1 ELISA Kit, PSF1 ELISA Kit, RING4 ELISA Kit, TAP1*0102N ELISA Kit, TAP1N ELISA Kit, abc17 ELISA Kit, abcb2 ELISA Kit, apt1 ELISA Kit, psf1 ELISA Kit, ring4 ELISA Kit, tap1a ELISA Kit, tap1n ELISA Kit, Abcb2 ELISA Kit, Ham-1 ELISA Kit, Ham1 ELISA Kit, MTP1 ELISA Kit, TAP ELISA Kit, Tap-1 ELISA Kit, Y3 ELISA Kit, Cim ELISA Kit, transporter 1, ATP binding cassette subfamily B member ELISA Kit, transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) L homeolog ELISA Kit, transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ELISA Kit, TAP1 ELISA Kit, tap1.L ELISA Kit, Tap1 ELISA Kit
- Hintergrund
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Gene Name: Antigen Peptide Transporter 1
Gene Aliases: APT1, ABC17, ABCB2, PSF1, RING4, Y3, TAP1N, Peptide Supply Factor 1, Atp-Binding Cassette,Subfamily B,Member 2, Peptide transporter involved in antigen processing 1
- Gen-ID
- 6890
- UniProt
- Q03518
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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