Angiotensin II Type-1 Receptor ELISA Kit

Produktnummer ABIN5651798

Produktdetails Angiotensin II Type-1 Receptor ELISA Kit

Target: Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Angiotensin II Receptor 1 (AGTR1). No significant cross-reactivity or interference between Angiotensin II Receptor 1 (AGTR1) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin II Receptor 1 (AGTR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin II Receptor 1 (AGTR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin II Receptor 1 (AGTR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin II Receptor 1 (AGTR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II Receptor 1 (AGTR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II Receptor 1 (AGTR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Angiotensin II Type-1 Receptor

Target: Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))

Andere Bezeichnung: Angiotensin II Receptor 1 (AGTR1 Produkte)

Synonyme: AG2S ELISA Kit, AGTR1A ELISA Kit, AGTR1B ELISA Kit, AT1 ELISA Kit, AT1AR ELISA Kit, AT1B ELISA Kit, AT1BR ELISA Kit, AT1R ELISA Kit, AT2R1 ELISA Kit, AT2R1A ELISA Kit, AT2R1B ELISA Kit, HAT1R ELISA Kit, 1810074K20Rik ELISA Kit, AI551199 ELISA Kit, AT1a ELISA Kit, Agtr-1a ELISA Kit, Agtr1 ELISA Kit, Angtr-1a ELISA Kit, AT1A ELISA Kit, XAT-1 ELISA Kit, agtr1-A ELISA Kit, agtr1.2 ELISA Kit, AT1-R ELISA Kit, at1 ELISA Kit, AGTR1 ELISA Kit, Agtr-1b ELISA Kit, Angtr-1b ELISA Kit, agtr1 ELISA Kit, agtr1-a ELISA Kit, agtr1-b ELISA Kit, xAT ELISA Kit, angiotensin II receptor type 1 ELISA Kit, angiotensin II receptor, type 1a ELISA Kit, angiotensin II receptor type 1 S homeolog ELISA Kit, uncharacterized AGTR1 ELISA Kit, angiotensin II receptor, type 1b ELISA Kit, angiotensin II receptor type 1 L homeolog ELISA Kit, AGTR1 ELISA Kit, Agtr1a ELISA Kit, agtr1.S ELISA Kit, Agtr1 ELISA Kit, Agtr1b ELISA Kit, agtr1.L ELISA Kit

Hintergrund:

Gene Name: Angiotensin II Receptor 1

Gene Aliases: AT1R, AG2S, AG2-S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2-R1, AT2R1A, AT2R1B, HAT1R, AT1 Receptor, Angiotensin Receptor 1B

Pathways: JAK-STAT Signalweg, ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour