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ACE2 ELISA Kit

ACE2 Reaktivität: Maus Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5651788
  • Target Alle ACE2 ELISA Kits anzeigen
    ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
    Reaktivität
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    12.5 pg/mL - 800 pg/mL
    Untere Nachweisgrenze
    12.5 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme 2 (ACE2). No significant cross-reactivity or interference between Angiotensin I Converting Enzyme 2 (ACE2) and analogues was observed.
    Sensitivität
    5.5 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme 2 (ACE2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin I Converting Enzyme 2 (ACE2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle ACE2 ELISA Kits anzeigen
    ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
    Andere Bezeichnung
    Angiotensin I Converting Enzyme 2 (ACE2 Produkte)
    Synonyme
    ACE1 ELISA Kit, CD143 ELISA Kit, DCP ELISA Kit, DCP1 ELISA Kit, ICH ELISA Kit, MVCD3 ELISA Kit, ACEH ELISA Kit, 2010305L05Rik ELISA Kit, zgc:92514 ELISA Kit, angiotensin I converting enzyme ELISA Kit, angiotensin I converting enzyme 2 ELISA Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 ELISA Kit, ACE ELISA Kit, ACE2 ELISA Kit, Ace2 ELISA Kit, ace2 ELISA Kit
    Hintergrund

    Gene Name: Angiotensin I Converting Enzyme 2

    Gene Aliases: ACEH, ACEII, ACE-II, Peptidyl-Dipeptidase A, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15

    Gen-ID
    70008
    UniProt
    Q8R0I0
    Pathways
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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