alpha 2 Antiplasmin ELISA Kit
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- Target Alle alpha 2 Antiplasmin (SERPINF2) ELISA Kits anzeigen
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 1.56 ng/mL - 100 ng/mL
- Untere Nachweisgrenze
- 1.56 ng/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Alpha-2-Plasmin Inhibitor (a2PI). No significant cross-reactivity or interference between Alpha-2-Plasmin Inhibitor (a2PI) and analogues was observed.
- Sensitivität
- 0.69 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Plasmin Inhibitor (a2PI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Plasmin Inhibitor (a2PI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Plasmin Inhibitor (a2PI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Plasmin Inhibitor (a2PI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Plasmin Inhibitor (a2PI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Plasmin Inhibitor (a2PI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle alpha 2 Antiplasmin (SERPINF2) ELISA Kits anzeigen
- alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))
- Andere Bezeichnung
- Alpha-2-Plasmin Inhibitor (SERPINF2 Produkte)
- Synonyme
- A2AP ELISA Kit, AAP ELISA Kit, ALPHA-2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, serpin ELISA Kit, AI747498 ELISA Kit, Pli ELISA Kit, Serpimf2 ELISA Kit, serpinf2 ELISA Kit, serpinf2l ELISA Kit, wu:fd57d08 ELISA Kit, Dmrs91 ELISA Kit, Pedf ELISA Kit, serpin family F member 2 ELISA Kit, serpin family F member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 2 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a ELISA Kit, SERPINF2 ELISA Kit, Serpinf2 ELISA Kit, SERPINF1 ELISA Kit, serpinf2a ELISA Kit, Serpinf1 ELISA Kit
- Hintergrund
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Gene Name: Alpha-2-Plasmin Inhibitor
Gene Aliases: SERPINF2, AAP, A2-PI, API, PLI, A2AP, Alpha 2 Antiplasmin, Serpin Peptidase Inhibitor Clade F Member 2, Pigment Epithelium Derived Factor
- Gen-ID
- 5345
- UniProt
- P08697
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones
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