Iba1 ELISA Kit

Produktnummer ABIN5651597

Produktdetails Iba1 ELISA Kit

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Allograft Inflammatory Factor 1 (AIF1). No significant cross-reactivity or interference between Allograft Inflammatory Factor 1 (AIF1) and analogues was observed.

Sensitivität: 6.4 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Allograft Inflammatory Factor 1 (AIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Allograft Inflammatory Factor 1 (AIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Allograft Inflammatory Factor 1 (AIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Allograft Inflammatory Factor 1 (AIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Allograft Inflammatory Factor 1 (AIF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Allograft Inflammatory Factor 1 (AIF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: Allograft Inflammatory Factor 1 (IBA1 Produkte)

Synonyme: AIF-1 ELISA Kit, IBA1 ELISA Kit, IRT-1 ELISA Kit, IRT1 ELISA Kit, AI607846 ELISA Kit, D17H6S50E ELISA Kit, G1 ELISA Kit, Iba1 ELISA Kit, Bart1 ELISA Kit, iba1 ELISA Kit, mrf-1 ELISA Kit, fa04b11 ELISA Kit, wu:fa04b11 ELISA Kit, AIF ELISA Kit, AIF1 ELISA Kit, aif1 ELISA Kit, allograft inflammatory factor 1 ELISA Kit, AIF1 ELISA Kit, Aif1 ELISA Kit, aif1 ELISA Kit

Hintergrund:

Gene Name: Allograft Inflammatory Factor 1

Gene Aliases: IBA1, IRT1, Daintain, Protein G1, Ionized calcium-binding adapter molecule 1

Gen-ID: 11629

UniProt: O70200

Pathways: Smooth Muscle Cell Migration