Smooth Muscle Actin ELISA Kit

Produktnummer ABIN5651363

Produktdetails Smooth Muscle Actin ELISA Kit

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 ng/mL - 500 ng/mL

Untere Nachweisgrenze: 7.8 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Actin Alpha 2, Smooth Muscle (ACTa2). No significant cross-reactivity or interference between Actin Alpha 2, Smooth Muscle (ACTa2) and analogues was observed.

Sensitivität: 2.9 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Alpha 2, Smooth Muscle (ACTa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Alpha 2, Smooth Muscle (ACTa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Alpha 2, Smooth Muscle (ACTa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Alpha 2, Smooth Muscle (ACTa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Alpha 2, Smooth Muscle (ACTa2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Alpha 2, Smooth Muscle (ACTa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Andere Bezeichnung: Actin Alpha 2, Smooth Muscle (ACTA2 Produkte)

Synonyme: AAT6 ELISA Kit, ACTSA ELISA Kit, MYMY5 ELISA Kit, GAMMA-ACTIN ELISA Kit, ACT ELISA Kit, ACTA3 ELISA Kit, ACTE ELISA Kit, ACTL3 ELISA Kit, ACTSG ELISA Kit, Act-4 ELISA Kit, Act4 ELISA Kit, SMGA ELISA Kit, 0610041G09Rik ELISA Kit, Actvs ELISA Kit, SMalphaA ELISA Kit, a-SMA ELISA Kit, alphaSMA ELISA Kit, wu:fb63d03 ELISA Kit, ASMA ELISA Kit, SMactin ELISA Kit, a-sma ELISA Kit, aat6 ELISA Kit, actsa ELISA Kit, mymy5 ELISA Kit, sm-actin ELISA Kit, ACTA2 ELISA Kit, actc1 ELISA Kit, ACT-4 ELISA Kit, asma ELISA Kit, ACTGE ELISA Kit, actin, alpha 2, smooth muscle, aorta ELISA Kit, actin, gamma 2, smooth muscle, enteric ELISA Kit, actin, alpha 2, smooth muscle, aorta L homeolog ELISA Kit, ACTA2 ELISA Kit, ACTG2 ELISA Kit, Actg2 ELISA Kit, Acta2 ELISA Kit, acta2 ELISA Kit, acta2.L ELISA Kit

Hintergrund:

Gene Name: Actin Alpha 2, Smooth Muscle

Gene Aliases: ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development