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ATR ELISA Kit

ATR Reaktivität: Human pThr1989 Colorimetric Sandwich ELISA Cell Culture Lysate
Produktnummer ABIN5526737
  • Target Alle ATR ELISA Kits anzeigen
    ATR (ATR serine/threonine kinase (ATR))
    Bindungsspezifität
    pThr1989, total
    Reaktivität
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-ATR (Thr1989) and Total ATR ELISA Kit. This assay semi-quantitatively measures ATR phosphorylated at Threonnine-1989 as well as total ATR in cell lysate samples.
    Proben
    Cell Culture Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes Human ATR phosphorylated at site Threonnine-1989 as well as total ATR.
    Produktmerkmale
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product ATR ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle ATR ELISA Kits anzeigen
    ATR (ATR serine/threonine kinase (ATR))
    Andere Bezeichnung
    ATR (ATR Produkte)
    Synonyme
    FCTCS ELISA Kit, FRP1 ELISA Kit, MEC1 ELISA Kit, SCKL ELISA Kit, SCKL1 ELISA Kit, ATR serine/threonine kinase ELISA Kit, ataxia telangiectasia and Rad3 related ELISA Kit, ATR ELISA Kit, Atr ELISA Kit
    Gen-ID
    545
    UniProt
    Q13535
    Pathways
    Positive Regulation of Response to DNA Damage Stimulus
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