GFP VHH, recombinant binding protein (GFP-Trap)

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Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
8
3
2
2
2
2
2
2
2
2
2
1
Wirt
Camelid (Camelidae)
Antikörpertyp
Recombinant Antibody
Applikation
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck For biochemical analyses of GFP fusion proteins.
Marke GFP-Trap®
Proben Cell Extracts
Kreuzreaktivität (Details) GFP-Trap® specifically binds to eGFP, wtGFP, GFP S65T, TagGFP, eYFP, YFP, Venus, Citrin, CFP. No binding to proteins derived from DsRed, all RFPs and TurboGFP can be detected.
Produktmerkmale Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.

Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).

Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.
Reinigung Purified protein
Bestandteile Alpaca anti-GFP VHH, purified unconjugated protein
Benötigtes Material Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
Protein- /Substanz
Andere Bezeichnung GFP
Hintergrund The green fluorescent protein (GFP) and variants thereof are widely used to study the subcellular localization and dynamics of proteins. GFP fusion proteins can be expressed in different cell types at different expression levels by transient or stable transfection. Transient expression may provide quick informative results, however, in many cases it is necessary to generate stable cell lines that express the GFP fusion protein of interest at a level similar to the one of the endogenous protein. Quantification of GFP fusion proteins in cells can be tricky since existing methods, like fluorescence microscopy or Western Blotting, are often shows insufficient signal to noise ratios or high signal variabilities .
Applikations-hinweise Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these GFP-fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via Immunoprecipitation using the GFP-Trap®. The GFP-Trap®_A enables purification of any protein of interest fused to GFP.
Testdauer 1.5 h
Protokoll
  • Robust and versatile tool for biochemical analyses of GFP-fusion proteins
  • Short incubation times (5 - 30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
  • Low unspecific binding
  • No contaminating heavy and light chains of conventional antibodies
  • Applicable in Chromatin Immunoprecipitation (ChIP)
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 µL resin (1mg/mL)
Buffer 1 x PBS,0.01% Sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Haltbarkeit 12 months
Bilder des Herstellers
 image for GFP VHH, recombinant binding protein (GFP-Trap) (ABIN509407) Left (IP): Pulldown of GFP with GFP-Trap®_A and GFP-Trap®_M from 293T cell extracts. ...
Western Blotting (WB) image for GFP VHH, recombinant binding protein (GFP-Trap) (ABIN509407) Comparison of GFP-Trap with conventional mono- and polyclonal antibodies: Immunopreci...
Produkt verwendet in: Morra, Del Carratore, Muhamadali, Horga, Halliwell, Goodacre, Breitling, Dixon: "Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins." in: mBio, Vol. 9, Issue 1, 2019 (PubMed).

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