RARS ELISA Kit
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- Target Alle RARS ELISA Kits anzeigen
- RARS (Arginyl-tRNA Synthetase (RARS))
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.781-50 ng/mL
- Untere Nachweisgrenze
- 0.781 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RARS in Mouse serum, plasma and other biological fluids
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This kit recognizes natural and recombinantMouseRARS. No significant cross-reactivity or interference between MouseRARS and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseRARS and all the analogues.
- Sensitivität
- 0.469 ng/mL
- Bestandteile
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Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis - Benötigtes Material
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Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer - Featured
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Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - Probenmenge
- 100 µL
- Plattentyp
- Pre-coated
- Protokoll
- This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RARS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RARSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RARS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of RARS.You can calculate the concentration of RARSin the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
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- Target Alle RARS ELISA Kits anzeigen
- RARS (Arginyl-tRNA Synthetase (RARS))
- Andere Bezeichnung
- Arginyl tRNA Synthetase (RARS) (RARS Produkte)
- Synonyme
- ArgRS ELISA Kit, CG9020 ELISA Kit, Dmel\\CG9020 ELISA Kit, RRS ELISA Kit, wu:fa96g09 ELISA Kit, zgc:56050 ELISA Kit, zgc:86730 ELISA Kit, RARS ELISA Kit, xcc-b100_4045 ELISA Kit, F28G11.14 ELISA Kit, F28G11_14 ELISA Kit, ECK1877 ELISA Kit, JW1865 ELISA Kit, lov ELISA Kit, 2610011N19Rik ELISA Kit, 2610037E21Rik ELISA Kit, AL033339 ELISA Kit, AW985894 ELISA Kit, DALRD1 ELISA Kit, ARGRS ELISA Kit, Arginyl-tRNA synthetase ELISA Kit, arginyl-tRNA synthetase ELISA Kit, hypothetical protein ELISA Kit, arginyL-tRNA synthetase ELISA Kit, Arginyl-tRNA synthetase, class Ic ELISA Kit, arginine--tRNA ligase ELISA Kit, ArgRS ELISA Kit, rars ELISA Kit, RARS ELISA Kit, ZMO0843 ELISA Kit, TP01_1024 ELISA Kit, Mmc1_0704 ELISA Kit, Oter_2566 ELISA Kit, argS ELISA Kit, TSTA_030410 ELISA Kit, PAAG_11057 ELISA Kit, MCYG_01632 ELISA Kit, Arch_0351 ELISA Kit, VDIS_RS05935 ELISA Kit, LOC100281742 ELISA Kit, Calkr_0529 ELISA Kit, Palpr_2492 ELISA Kit, Mspyr1_17280 ELISA Kit, AT1G66530 ELISA Kit, UCYN_05320 ELISA Kit, Rars ELISA Kit
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