NLRC4 ELISA Kit

Produktnummer ABIN4993600

Produktdetails NLRC4 ELISA Kit

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.13 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.13 pg/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This kit recognizes natural and recombinantHumanNLRC4. No significant cross-reactivity or interference between HumanNLRC4 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanNLRC4 and all the analogues.

Sensitivität: 46.88 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Anwendungsinformationen

Kommentare:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Probenmenge: 100 µL

Plattentyp: Pre-coated

Protokoll:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Aufbereitung der Reagenzien:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 5000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 5000, 2500, 1250, 625, 312.5, 156.25, 78.13, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 5000 pg/mL working solution to the first tube and mix up to produce a 2500 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Details zu NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Andere Bezeichnung: NLR family CARD domain-containing protein 4 (NLRC4) (NLRC4 Produkte)

Synonyme: CARD12 ELISA Kit, CLAN ELISA Kit, CLAN1 ELISA Kit, CLANA ELISA Kit, CLANB ELISA Kit, CLANC ELISA Kit, CLAND ELISA Kit, CLR2.1 ELISA Kit, IPAF ELISA Kit, 9530011P19Rik ELISA Kit, Card12 ELISA Kit, NLR family CARD domain containing 4 ELISA Kit, NLR family, CARD domain containing 4 ELISA Kit, NLRC4 ELISA Kit, Nlrc4 ELISA Kit

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome