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HES1 ELISA Kit (Hes Family bHLH Transcription Factor 1) ELISA Kit

HES1 Reaktivität: Human Colorimetric Sandwich ELISA 78.125-5000 pg/mL Plasma
Produktnummer ABIN4993496
$630.00
Zzgl. Versandkosten $45.00
96 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 12 bis 14 Werktagen
  • Target
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.125-5000 pg/mL
    Untere Nachweisgrenze
    78.125 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HES1 in human serum, plasma and other biological fluids
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This kit recognizes natural and recombinantHumanHES1. No significant cross-reactivity or interference between HumanHES1 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanHES1 and all the analogues.
    Sensitivität
    46.875 pg/mL
    Bestandteile
    Micro ELISA Plate
    Reference Standard
    Concentrated Biotinylated Detection Ab
    Concentrated HRP Conjugate
    Reference Standard & Sample Diluent
    Biotinylated Detection Ab Diluent
    HRP Conjugate Diluent
    Concentrated Wash Buffer (25×)
    Substrate Reagent
    Stop Solutio
    Plate Sealer
    Product Description
    Certificate of Analysis
    Benötigtes Material
    Microplate reader with 450nm wavelength filter
    High-precision transferpettor, EP tubes and disposable pipette tips
    37℃ Incubator
    Deionized or distilled water
    Absorbent paper
    Loading slot for Wash Buffer
  • Kommentare

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    Probenmenge
    100 µL
    Plattentyp
    Pre-coated
    Protokoll
    This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HES1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HES1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HES1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HES1.You can calculate the concentration of HES1in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • Target
    Andere Bezeichnung
    Transcription factor HES-1 (HES1) (HES1 ELISA Kit Abstract)
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