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Glutathione Reductase ELISA Kit

GSR Reaktivität: Ratte Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum
Produktnummer ABIN4993476
  • Target Alle Glutathione Reductase (GSR) ELISA Kits anzeigen
    Glutathione Reductase (GSR)
    Reaktivität
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    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.63 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    15.63 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This kit recognizes natural and recombinantRatGR. No significant cross-reactivity or interference between RatGR and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatGR and all the analogues.
    Sensitivität
    9.38 pg/mL
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Reference Standard & Sample Diluent
    • Biotinylated Detection Antibody (100 x concentrate)
    • HRP Conjugate (100 x concentrate)
    • Biotinylated Detection Antibody Diluent
    • HRP Conjugate Diluent
    • Substrate Reagent
    • Stop Solution
    • Wash Buffer (25 x concentrate)
    • Instruction manual
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  • Kommentare

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    Probenmenge
    100 µL
    Plattentyp
    Pre-coated
    Protokoll
    1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
    2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
    3. Aspirate and wash 3 times.
    4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
    5. Aspirate and wash 5 times.
    6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
    7. Add 50 µL Stop Solution. Read at 450 nm immediately.
    8. Calculation of results.
    Aufbereitung der Reagenzien
    1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
    2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
    3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 1000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 1000, 500, 250, 125, 62.5, 31.25, 15.63, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 1000 pg/mL working solution to the first tube and mix up to produce a 500 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
    4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
    5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • Target Alle Glutathione Reductase (GSR) ELISA Kits anzeigen
    Glutathione Reductase (GSR)
    Andere Bezeichnung
    Glutathione Reductase (GR) (GSR Produkte)
    Synonyme
    GR ELISA Kit, ATGR2 ELISA Kit, EMB2360 ELISA Kit, glutathione reductase ELISA Kit, AI325518 ELISA Kit, D8Ertd238e ELISA Kit, Gr-1 ELISA Kit, Gr1 ELISA Kit, DDBDRAFT_0168952 ELISA Kit, DDBDRAFT_0231410 ELISA Kit, DDB_0168952 ELISA Kit, DDB_0231410 ELISA Kit, 2151 ELISA Kit, CG2151 ELISA Kit, DTR ELISA Kit, Dm-TrxR ELISA Kit, DmTR ELISA Kit, DmTrx ELISA Kit, DmTrxR ELISA Kit, DmTrxR-1 ELISA Kit, Dmel\\CG2151 ELISA Kit, Gr ELISA Kit, Trx ELISA Kit, TrxR ELISA Kit, TrxR-1 ELISA Kit, Trxr1 ELISA Kit, anon-WO03040301.185 ELISA Kit, anon-WO03040301.187 ELISA Kit, dTrxR ELISA Kit, dmtrxr-1 ELISA Kit, gr ELISA Kit, l(1)G0154 ELISA Kit, l(1)G0379 ELISA Kit, l(1)G0477 ELISA Kit, l(1)G0481 ELISA Kit, trxr-1 ELISA Kit, gor1 ELISA Kit, glutathione reductase, gro-2 ELISA Kit, glutathione reductase ELISA Kit, glutathione-disulfide reductase ELISA Kit, GR; GRase ELISA Kit, mitochondrial glutathione reductase Pgr1 ELISA Kit, Glutathione reductase (GR) (GRase) ELISA Kit, Thioredoxin reductase-1 ELISA Kit, glutathione-disulfide reductase family protein ELISA Kit, glutathione reductase 1 ELISA Kit, GR ELISA Kit, gor ELISA Kit, GSR ELISA Kit, GSR1 ELISA Kit, Gsr_2 ELISA Kit, Gsr ELISA Kit, GSHR1 ELISA Kit, gsr ELISA Kit, LbGR ELISA Kit, pgr1 ELISA Kit, GSHR2 ELISA Kit, GLR2 ELISA Kit, Bcen_2392 ELISA Kit, RPE_1989 ELISA Kit, HCAG_02219 ELISA Kit, SJAG_01162 ELISA Kit, Trxr-1 ELISA Kit, PF14_0192 ELISA Kit, POPTR_0001s14480g ELISA Kit, gsr1 ELISA Kit
    Pathways
    Thyroid Hormone Synthesis, Cell RedoxHomeostasis
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