FGF10 ELISA Kit

Produktnummer ABIN4993446

Produktdetails FGF10 ELISA Kit

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.81 pg/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGF10 in human serum, plasma and other biological fluids

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This kit recognizes natural and recombinantHumanFGF10. No significant cross-reactivity or interference between HumanFGF10 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanFGF10 and all the analogues.

Sensitivität: 4.69 pg/mL

Bestandteile: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Benötigtes Material: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Anwendungsinformationen

Kommentare:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Probenmenge: 100 µL

Plattentyp: Pre-coated

Protokoll: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF10. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FGF10and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FGF10.You can calculate the concentration of FGF10in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Details zu FGF10

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Abstract: FGF10 Produkte

Synonyme: fgf10 ELISA Kit, fgf-10 ELISA Kit, wu:fd11d03 ELISA Kit, zgc:109774 ELISA Kit, FGF10 ELISA Kit, AEY17 ELISA Kit, BB213776 ELISA Kit, Fgf-10 ELISA Kit, Gsfaey17 ELISA Kit, FGF-10 ELISA Kit, fibroblast growth factor 10a ELISA Kit, fibroblast growth factor 10 ELISA Kit, fgf10a ELISA Kit, FGF10 ELISA Kit, fgf10 ELISA Kit, Fgf10 ELISA Kit

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus