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TR4 ELISA Kit

NR2C2 Reaktivität: Human pSer412 Colorimetric Sandwich ELISA Cell Culture Lysate
Produktnummer ABIN4886315
  • Target Alle TR4 (NR2C2) ELISA Kits anzeigen
    TR4 (NR2C2) (Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))
    Bindungsspezifität
    pSer412, total
    Reaktivität
    • 4
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-TAK1 (Ser412) and Total TAK1 ELISA Kit. This assay semi-quantitatively measures phophorylated TAK1 (Ser412) and Total TAK1 in lysate samples.
    Proben
    Cell Culture Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes Human TAK1 phosphorylated at site Serine-412 as well as total TAK1.
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle TR4 (NR2C2) ELISA Kits anzeigen
    TR4 (NR2C2) (Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))
    Andere Bezeichnung
    TAK1 (NR2C2 Produkte)
    Synonyme
    TAK1 ELISA Kit, TR2R1 ELISA Kit, TR4 ELISA Kit, hTAK1 ELISA Kit, Tr4 ELISA Kit, gb:dq017625 ELISA Kit, im:6911408 ELISA Kit, mKIAA4145 ELISA Kit, nuclear receptor subfamily 2 group C member 2 ELISA Kit, nuclear receptor subfamily 2, group C, member 2 ELISA Kit, NR2C2 ELISA Kit, Nr2c2 ELISA Kit, nr2c2 ELISA Kit
    Gen-ID
    6885
    UniProt
    O43318
    Pathways
    T-Zell Rezeptor Signalweg, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Toll-Like Receptors Cascades
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