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JAK1 ELISA Kit

JAK1 Reaktivität: Human, Ratte, Maus pThr246 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886155
  • Target Alle JAK1 ELISA Kits anzeigen
    JAK1 (Janus Kinase 1 (JAK1))
    Bindungsspezifität
    pThr246, total
    Reaktivität
    Human, Ratte, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-PRAS40 (T246) & Total PRAS40 ELISA Kit. This assay semi-quantitatively measures phophorylated PRAS40 (Thr246) & Total PRAS40 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human JAK1 phosphorylated at site Tyrosine-1022 and total JAK1
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product JAK1 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Al-Maghrebi, Renno, Al-Somali, Botras, Qadhi: "Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 389, Issue 5, pp. 539-51, (2017) (PubMed).

  • Target Alle JAK1 ELISA Kits anzeigen
    JAK1 (Janus Kinase 1 (JAK1))
    Andere Bezeichnung
    JAK1 (JAK1 Produkte)
    Synonyme
    JAK1A ELISA Kit, JAK1B ELISA Kit, JTK3 ELISA Kit, AA960307 ELISA Kit, C130039L05Rik ELISA Kit, wu:fb93e10 ELISA Kit, jak1 ELISA Kit, Janus kinase 1 ELISA Kit, tyrosine-protein kinase JAK1 ELISA Kit, JAK1 ELISA Kit, Jak1 ELISA Kit, jak1 ELISA Kit, LOC100286690 ELISA Kit
    Gen-ID
    3716
    UniProt
    P23458
    Pathways
    JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded Protein Response
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