TR4 ELISA Kit

Produktnummer ABIN4885975

Produktdetails TR4 ELISA Kit

Target: TR4 (NR2C2) (Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))

Bindungsspezifität: pThr69, pThr71

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human and Mouse Phospho-ATF2 (Thr69/71) ELISA Kit. This assay semi-quantitatively measures phophorylated ATF2 (Thr69/71) in lysate samples.

Proben: Cell Culture Lysate

Analytische Methode: Semi-Quantitative

Spezifität: This ELISA kit recognizes Human and Mouse TAK1 phosphorylated at site Threonine-69/71.

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu TR4

Target: TR4 (NR2C2) (Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))

Andere Bezeichnung: TAK1 (NR2C2 Produkte)

Synonyme: TAK1 ELISA Kit, TR2R1 ELISA Kit, TR4 ELISA Kit, hTAK1 ELISA Kit, Tr4 ELISA Kit, gb:dq017625 ELISA Kit, im:6911408 ELISA Kit, mKIAA4145 ELISA Kit, nuclear receptor subfamily 2 group C member 2 ELISA Kit, nuclear receptor subfamily 2, group C, member 2 ELISA Kit, NR2C2 ELISA Kit, Nr2c2 ELISA Kit, nr2c2 ELISA Kit

Gen-ID: 1386

UniProt: P15336, P16951

Pathways: T-Zell Rezeptor Signalweg, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Toll-Like Receptors Cascades