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SPOCK3 ELISA Kit

SPOCK3 Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885655
  • Target Alle SPOCK3 ELISA Kits anzeigen
    SPOCK3 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
    Reaktivität
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Mouse Testican 3 (SPOCK) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes mouse Testican 3/SPOCK.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle SPOCK3 ELISA Kits anzeigen
    SPOCK3 (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
    Andere Bezeichnung
    Testican 3 (SPOCK3 Produkte)
    Synonyme
    im:7139568 ELISA Kit, si:dkey-285b22.1 ELISA Kit, HSAJ1454 ELISA Kit, TES-3 ELISA Kit, TICN3 ELISA Kit, Testican-3 ELISA Kit, 2900045C01Rik ELISA Kit, AI428471 ELISA Kit, mKIAA4039 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3 ELISA Kit, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 ELISA Kit, spock3 ELISA Kit, SPOCK3 ELISA Kit, Spock3 ELISA Kit
    Gen-ID
    72902
    UniProt
    Q8BKV0
    Pathways
    Glycosaminoglycan Metabolic Process
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