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SPOCK3 ELISA Kit
SPOCK3
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885655
Produktdetails SPOCK3 ELISA Kit
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Target
Alle SPOCK3 ELISA Kits anzeigen
SPOCK3
(Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
Reaktivität
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Mouse Testican 3 (SPOCK) ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes mouse Testican 3/SPOCK.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SPOCK3
(ausblenden)
Target
Alle SPOCK3 ELISA Kits anzeigen
SPOCK3
(Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 3 (SPOCK3))
Andere Bezeichnung
Testican 3 (SPOCK3 Produkte )
Synonyme
im:7139568 ELISA Kit, si:dkey-285b22.1 ELISA Kit, HSAJ1454 ELISA Kit, TES-3 ELISA Kit, TICN3 ELISA Kit, Testican-3 ELISA Kit, 2900045C01Rik ELISA Kit, AI428471 ELISA Kit, mKIAA4039 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3 ELISA Kit, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3 ELISA Kit, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 ELISA Kit, spock3 ELISA Kit, SPOCK3 ELISA Kit, Spock3 ELISA Kit
Gen-ID
72902
UniProt
Q8BKV0
Pathways
Glycosaminoglycan Metabolic Process
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