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PGLYRP1 ELISA Kit
PGLYRP1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4885318
Produktdetails PGLYRP1 ELISA Kit
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Target
Alle PGLYRP1 ELISA Kits anzeigen
PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Reaktivität
Alle Reaktivitäten für PGLYRP1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human PGRPs SpeedELISA Kit.
Marke
SpeedELISA
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Produktmerkmale
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Testdauer
3 h
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Haltbarkeit
6 months
Details zu PGLYRP1
(ausblenden)
Target
Alle PGLYRP1 ELISA Kits anzeigen
PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Andere Bezeichnung
PGRPs (PGLYRP1 Produkte )
Synonyme
PGLYRP1 ELISA Kit, PGLYRP ELISA Kit, pgrp ELISA Kit, tag7 ELISA Kit, pgrps ELISA Kit, pglyrp ELISA Kit, pgrp-s ELISA Kit, tnfsf3l ELISA Kit, MGC108330 ELISA Kit, MGC108469 ELISA Kit, PGRP-SC1a ELISA Kit, si:dkey-113g17.4 ELISA Kit, si:ch211-202c21.3 ELISA Kit, PGRP ELISA Kit, PGRP-S ELISA Kit, PGRPS ELISA Kit, TAG7 ELISA Kit, TNFSF3L ELISA Kit, Pglyrp ELISA Kit, Tag7 ELISA Kit, Tasg7 ELISA Kit, Tnfsf3l ELISA Kit, Pgrp ELISA Kit, peptidoglycan recognition protein 1 ELISA Kit, peptidoglycan recognition protein 1 L homeolog ELISA Kit, peptidoglycan recognition protein 5 ELISA Kit, PGLYRP1 ELISA Kit, pglyrp1.L ELISA Kit, pglyrp1 ELISA Kit, pglyrp5 ELISA Kit, Pglyrp1 ELISA Kit
Gen-ID
8993
UniProt
O75594
Pathways
Activation of Innate immune Response
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