FETUB ELISA Kit
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- Target Alle FETUB ELISA Kits anzeigen
- FETUB (Fetuin B (FETUB))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Custom Human Fetuin B SpeedELISA Kit.
- Marke
- SpeedELISA
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Produktmerkmale
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Probenmenge
- 100 μL
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - Haltbarkeit
- 6 months
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- Target Alle FETUB ELISA Kits anzeigen
- FETUB (Fetuin B (FETUB))
- Andere Bezeichnung
- Fetuin B (FETUB Produkte)
- Synonyme
- 16G2 ELISA Kit, Gugu ELISA Kit, IRL685 ELISA Kit, Fet ELISA Kit, Pp63 ELISA Kit, gugu ELISA Kit, fetuin-b ELISA Kit, FETUB ELISA Kit, 16g2 ELISA Kit, irl685 ELISA Kit, im:6910148 ELISA Kit, 2310011O17Rik ELISA Kit, AI255764 ELISA Kit, D17980 ELISA Kit, fetuin B ELISA Kit, fetuin B L homeolog ELISA Kit, fetuin beta ELISA Kit, FETUB ELISA Kit, Fetub ELISA Kit, fetub.L ELISA Kit, fetub ELISA Kit
- Gen-ID
- 26998
- UniProt
- Q9UGM5
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