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VHL ELISA Kit
VHL
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885037
Produktdetails VHL ELISA Kit
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Target
Alle VHL ELISA Kits anzeigen
VHL
(Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))
Reaktivität
Alle Reaktivitäten für VHL ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human VHL ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Discover our top product VHL ELISA Kit
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu VHL
(ausblenden)
Target
Alle VHL ELISA Kits anzeigen
VHL
(Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))
Andere Bezeichnung
VHL (VHL Produkte )
Synonyme
HRCA1 ELISA Kit, RCA1 ELISA Kit, VHL1 ELISA Kit, pVHL ELISA Kit, BcDNA:RH61560 ELISA Kit, CG13221 ELISA Kit, DVhl ELISA Kit, Dmel\\CG13221 ELISA Kit, Dvhl ELISA Kit, VHL ELISA Kit, d-VHL ELISA Kit, d-vhl ELISA Kit, dVHL ELISA Kit, dmVHL ELISA Kit, vhl ELISA Kit, rca1 ELISA Kit, vhl1 ELISA Kit, hrca1 ELISA Kit, zgc:158722 ELISA Kit, Vhlh ELISA Kit, von Hippel-Lindau tumor suppressor ELISA Kit, von Hippel-Lindau ELISA Kit, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase ELISA Kit, von Hippel-Lindau disease tumor suppressor ELISA Kit, VHL ELISA Kit, Vhl ELISA Kit, vhl ELISA Kit, CpipJ_CPIJ009992 ELISA Kit
Gen-ID
7428
UniProt
P40337
Pathways
Tube Formation , Signaling Events mediated by VEGFR1 and VEGFR2 , Ubiquitin Proteasome Pathway
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